Supplementation of Chlorella vulgaris meal in diet for growth performance of striped catfish juvenile Pangasianodon hypophthalmus

Abstract

The culture of striped catfish (Pangasianodon hypophthalmus) is overgrowing and facing the problem of relatively high feed prices. The feed prices affect feed grade; the cheaper the feed price, the lower the quality. This low feed quality may improve its quality by supplementing Chlorella vulgaris. Therefore, this study aimed to determine the level of supplementation of C. vulgaris meal in the feed that produces the best growth performance in striped catfish juveniles. A completely randomized design with three treatments and three replications was used in this experiment. The treatments were various levels of C. vulgaris, namely 0%, 1.5%, and 3%. C. vulgaris meal was added to the commercial feed (26-28% protein) according to the treatment with the coating method. The test feed was fed to striped catfish juveniles (6.66 ± 0.04 g in average weight) reared in an aquarium containing 55 L of water at a density of 20 juveniles, at satiation in the morning, afternoon, and evening for 50 days of the rearing period. Survival rate and hepatosomatic indices of the striped catfish were not affected by various supplementation levels of C. vulgaris meal (P>0.05), but the values of feed consumption, specific growth rate, feed conversion, and protein efficiency ratio of the striped catfish (P<0.05). Supplementation of 3.5% C. vulgaris meal in feed resulted in the best growth performance for striped catfish.

References

Abdel-Tawwab, M., Abdulrahman, N. M., Baiz, A. I., Nader, P. J., & Al-Refaiee, I. H. A. (2022). The using of Chlorella pyrenoidosa and Daphnia magna as feed supplements for common carp, Cyprinus carpio: growth performance, somatic indices, and hemato-biochemical biomarkers. Journal of Applied Aquaculture, 34(1), 64–78. doi: 10.1080/10454438.2020.1787291.

Ahmad, A., W. Hassan, S., & Banat, F. (2022). An overview of microalgae biomass as a sustainable aquaculture feed ingredient: food security and circular economy. Bioengineered, 13(4), 9521–9547. doi: 10.1080/21655979.2022.2061148.

Ahmad, M., Shariff, M., Md. Yusoff, F., Goh, Y., & Banerjee, S. (2020). Applications of microalga Chlorella vulgaris in aquaculture. Reviews in Aquaculture, 12(1), 328–346. doi: 10.1111/raq.12320.

An, B. K., Kim, K. E., Jeon, J. Y., & Lee, K. W. (2016). Effect of dried Chlorella vulgaris and chlorella growth factor on growth performance, meat qualities and humoral immune responses in broiler chickens. SpringerPlus, 5, 718–724. doi: 10.1186/s40064-016-2373-4.

Badan Standarisasi Nasional Indonesia. (2000). SNI 01-6483.4-2000:Produksi benih ikan patin siam (Pangasius hypophthalmus) kelas benih sebar. Jakarta. BSNI.

Barone, R. S. C., Sonoda, D. Y., Lorenz, E. K., & Cyrino, J. E. P. (2018). Digestibility and pricing of Chlorella sorokiniana meal for use in tilapia feeds. Scientia Agricola, 75(3), 184–190. doi: 10.1590/1678-992x-2016-0457.

Camacho-Rodríguez, J., Macías-Sánchez, M. D., Cerón-García, M. C., Alarcón, F. J., & Molina-Grima, E. (2018). Microalgae as a potential ingredient for partial fish meal replacement in aquafeeds: nutrient stability under different storage conditions. Journal of Applied Phycology, 30(2), 1049–1059. doi: 10.1007/s10811-017-1281-5.

Enyidi, U. D. (2017). Chlorella vulgaris as protein source in the diets of African catfish Clarias gariepinus. Fishes, 2(4), 17. doi: 10.3390/fishes2040017.

Erturk, M. M., & Sevgili, H. (2003). Effects of replacement of fish meal with poultry by-product meals on apparent digestibility, body composition and protein efficiency ratio in a practical diets for rainbow trout, Onchorynchus mykiss. Asian-Australasian Journal of Animal Sciences, 16(9), 1355–1359. doi: 10.5713/ajas.2003.1355.

Hana, H., & Simanjuntak, S. B. I. (2021). Indeks morfoanatomi ikan lele dumbo (Clarias gariepinus) yang diberi suplementasi pakan Spirulina platensis dan Chlorella vulgaris. Pengembangan Sumber Daya Perdesaan dan Kearifan Lokal Berkelanjutan XI, 61–77.

Hasan, M. R., & Shipton, T. A. (2021). Aquafeed value chain analysis of striped catfish in Vietnam. Aquaculture, 541, 736798. doi: 10.1016/j.aquaculture.2021.736798

Ighwela, K. A., Terengganu, K., Ahmad, A. Bin, & Abol-Munafi, A. B. (2014). The selection of viscerosomatic and hepatosomatic indices for the measurement and analysis of Oreochromis niloticus condition fed with varying dietary maltose levels. International Journal of Fauna and Biological Studies, 1(13), 18–20.

Khani, M., Soltani, M., Shamsaie Mehrjan, M., Foroudi, F., & Ghaeni, M. (2017a). The effect of Chlorella vulgaris (Chlorophyta, Volvocales) microalga on some hematological and immune system parameters of Koi carp (Cyprinus carpio). Iranian Journal of Ichthyology, 4(1), 62–68. doi: 10.7508/iji.2016.02.015.

Khani, M., Soltani, M., Shamsaie Mehrjan, M., Foroudi, F., & Ghaeni, M. (2017b). The effects of Chlorella vulgaris supplementation on growth performance, blood characteristics, and digestive enzymes in Koi (Cyprinus carpio). Iranian Journal of Fisheries Sciences, 16(2), 832–843.

Kim, S. S., Rahimnejad, S., Kim, K. W., & Lee, K. J. (2013). Partial replacement of fish meal with Spirulina pacifica in diets for parrot fish (Oplegnathus fasciatus). Turkish Journal of Fisheries and Aquatic Sciences, 13, 197–204. doi: 10.4194/1303-2712-v13_2_01.

Li, J., Fan, Z., Qu, M., Qiao, X., Sun, J., Bai, D., & Cheng, Z. (2015). Applications of microalgae as feed additives in aquaculture. International Symposium on Energy Science and Chemical Engineering, 352–356. doi: 10.2991/isesce-15.2015.72.

Nagappan, S., Das, P., AbdulQuadir, M., Thaher, M., Khan, S., Mahata, C., Al-Jabri, H., Vatland, A. K., & Kumar, G. (2021). Potential of microalgae as a sustainable feed ingredient for aquaculture. Journal of Biotechnology, 341, 1–20. doi: 10.1016/j.jbiotec.2021.09.003.

Nates, S. F. (2015). Aquafeed Formulation. New York: Academic Press. doi: 10.1016/C2013-0-18878-2.

Nawawi, A., Lumbessy, S., & Damayanti, A. (2015). Pengaruh frekuensi pemberian pakan terhadap pertumbuhan dan kelangsungan hidup benih ikan patin Pangasius sp. Jurnal Perikanan Unram, 6, 42–48. doi: 10.29303/jp.v6i1.49.

Purnomo, N., Utomo, N. B. P., & Azwar, Z. I. (2015). The growth and meat quality of Siamese catfish fed different level of protein. Jurnal Akuakultur Indonesia, 14(2), 104–111. doi: 10.19027/jai.14.104-111.

Raji, A. A., Alaba, P. A., Yusuf, H., Abu Bakar, N. H., Mohd Taufek, N., Muin, H., Alias, Z., Milow, P., & Abdul Razak, S. (2018). Fishmeal replacement with Spirulina platensis and Chlorella vulgaris in African catfish (Clarias gariepinus) diet: Effect on antioxidant enzyme activities and haematological parameters. Research in Veterinary Science, 119, 67–75. doi: 10.1016/j.rvsc.2018.05.013.

Setiawati, M., Jusadi, D., Rolin, F., & Vinasyiam, A. (2016). Evaluasi pemberian ekstrak daun kayu manis Cinnamomum burmannii pada pakan terhadap kandungan lemak daging ikan patin Pangasianodon hypopthalmus. Jurnal Akuakultur Indonesia, 15(2), 132–138. doi: 10.19027/jai.15.2.132-138.

Shah, M. R., Lutzu, G. A., Alam, A., Sarker, P., Kabir Chowdhury, M. A., Parsaeimehr, A., Liang, Y., & Daroch, M. (2018). Microalgae in aquafeeds for a sustainable aquaculture industry. Journal of Applied Phycology, 30(1), 197–213. doi: 10.1007/s10811-017-1234-z.

Shalihah, H., Munandar, A., & Syamsunarno, M. B. (2019). Utilization of rubber seed oil in diet for supporting growth performance of common carp Cyprinus carpio. Depik, 8(1), 26–35. doi: 10.13170/depik.8.1.13100.

Simanjuntak, S. B. I. (2020). The discontinuous feeding effects of Chlorella vulgaris supplemented feed on the gourami body composition. IOP Conference Series: Earth and Environmental Science, 593(1), 012018. doi: 10.1088/1755-1315/593/1/012018.

Simanjuntak, S. B. I, Indarmawan, & Wibowo, E. S. (2019). Pengaruh pakan suplementasi Spirulina platensis dan Chlorella vulgaris terhadap pertumbuhan dan komposisi tubuh ikan gurami (Osphronemus gouramy). Majalah Ilmiah Biologi Biosfera: A Scientific Journal, 36(2), 51–56. doi: 10.20884/1.mib.2019.36.2.724.

Simanjuntak, S. B. I, Hana, Yunida, R., & Zuwanda, M. (2022). Lipase activity, hematological and blood biochemistry of Osphronemus gouramy fed with suplementation of Spirulina platensis and Chlorella vulgaris. Molekul, 17(1), 85–97.

Sugiharto, S. (2020). Chlorella vulgaris and Spirulina platensis: Their nutrient contents and bioactive compounds for improving poultry productivity. Wartazoa, 30(3), 123–138. doi: 10.14334/wartazoa.v30i3.2523.

Sunarno, M. T. .D, Sulhi, M., Suryaningrum, L. M., & Wilakstanti, M. (2014). Penelitian pengembangan pakan efisien dan ekonomis berbasis bahan baku lokal untuk budidaya ikan patin (Pangasionodon hypopthalmus) di Kabupaten Kampar, Riau. Prosiding Forum Inovasi Teknologi Akuakultur 2014, 587–595.

Sunarno, M. T. D., Sulhi, M., & Suryaningrum, L. M. (2013). Kajian pabrik pakan ikan lokal dalam mendukung industrialisasi budidaya ikan patin (Pangasius sp .) di Kabupaten Kampar Provinsi Riau. Prosiding Forum Inovasi Teknologi Akuakultur 2013, 371–379.

Sunarno, M. T. D., & Marson. (2012). Swampy area for development of patin culture. Proceeding International Conference on Indonesia Inland Waters III, 57–63.

Syamsunarno, M. B. S., & Sunarno, M. T. D. (2016). Budidaya ikan air tawar ramah lingkungan untuk mendukung keberlanjutan penyediaan ikan bagi masyarakat. Seminar Nasional Perikanan dan Kelautan 2016, 1–16.

Teuling, E., Schrama, J. W., Gruppen, H., & Wierenga, P. A. (2017). Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarias gariepinus). Aquaculture, 479, 490–500. doi: 10.1016/j.aquaculture.2017.06.025.

Utomo, N. B. P., Rahmatia, F., & Setiawan, M. (2012). Penggunaan Spirulina platensis sebagai suplemen bahan baku pakan ikan nila Oreochromis niloticus. Jurnal Akuakultur Indonesia, 11(1), 49–53.

Widiyati, A., & Sunarno, M. T. D. (2016). Pembesaran ikan patin (Pangasionodon hypopthalmus) dengan pakan berbasis bahan baku lokal pada kandungan proten berbeda. Prosiding Forum Inovasi Teknologi Akuakultur 2016, 643–652.

Xu, W., Gao, Z., Qi, Z., Qiu, M., Peng, J. Q., & Shao, R. (2014). Effect of dietary chlorella on the growth performance and physiological parameters of gibel carp, Carassius auratus gibelio. Turkish Journal of Fisheries and Aquatic Sciences, 14(1), 53–57. doi: 10.4194/1303-2712-v14_1_07.

Zakiah, K. S., Simanjuntak, S. B. I., & Wibowo, E. S. (2020). The effect of suplementation Chlorella vulgaris on feed to albumin levels in serum nilem fish (Osteochilus vittatus). BioEksakta, 2(3), 313–320. doi: 10.20884/1.bioe.2020.2.3.2144.