Isolation and Identification of Bacteria from the Kidney, Gills, and Skin Mucus of Mud Grouper (Epinephelus coioides) Cultured in Floating Net Cages in Lhokseumawe
DOI:
https://doi.org/10.51179/jsp.v9i3.3722Keywords:
API Kit, Epinephelus coioides, bacterial identification, floating net cagesAbstract
Bacterial diseases are among the major factors limiting the productivity of mud grouper (Epinephelus coioides) aquaculture. This study aimed to isolate and identify bacteria from the kidney, gills, and skin mucus of mud grouper cultured in floating net cages in Lhokseumawe, Aceh, Indonesia. The study was conducted from August to September 2024 using an observational method combined with laboratory-based analysis. A total of ten fish (approximately 20 cm in length) showing clinical signs of bacterial infection, including lethargy, erythema, and hemorrhage, were selected using purposive sampling. Bacterial isolation was performed on 3% Tryptic Soy Agar (TSA) and incubated at 26–30°C for 24 h. The isolates were identified using the Analytical Profile Index (API) Kit. The results revealed the presence of Vibrio alginolyticus, Vibrio harveyi, Streptococcus iniae, and Bacillus cereus. Vibrio alginolyticus and Vibrio harveyi were isolated from the kidney and gills, whereas Streptococcus iniae was detected in the gills and skin mucus. Bacillus cereus was identified only from the skin mucus. These findings provide baseline information on bacterial diversity associated with cultured mud grouper and may support disease diagnosis and fish health management in floating net cage aquaculture.
References
Austrian, R., Rozi, R., & Rahayu, K. (2021). Efektivitas penggunaan API 20E kit untuk mengidentifikasi bakteri Gram negatif pada komoditas perikanan laut. Jurnal Ilmiah Perikanan dan Kelautan, 13(2), 188-195. https://doi.org/10.20473/jipk.v13i2.24610
Fauzi, M., Amelia, R., & Siregar, T. (2021). Prospek ekonomi dan pengembangan budidaya ikan kerapu lumpur (Epinephelus coioides) di perairan pantai. Jurnal Kebijakan Perikanan, 13(1), 45-53. https://doi.org/10.15578/jkp.13.1.2021.45-53
Hasmar, H., Rosmawati, R., & Hidayat, S. (2022). Peran mukus kulit sebagai sistem pertahanan non-spesifik pada ikan laut terhadap infeksi bakteri. Jurnal Akuatika Indonesia, 7(2), 112-119. https://doi.org/10.24198/jaki.v7i2.39412
Irianto, A., Kuswanta, F., & Prayitno, S. B. (2021). Patologi dan transmisi bakteri patogen pada ikan laut ekonomis penting. Jurnal Patologi Perikanan, 9(3), 201-210. https://doi.org/10.29303/jpat.v9i3.412
Izwar, A., Fauza, S., Muktitama, A. M., Nugrahawati, A., Azhar, R., Putra, D. P., ... & Musarady, Y. (2025). The water quality and biological indicators of estuarine and marine waters affected by shrimp farming effluents in Bireuen Regency, Aceh. Acta Aquatica: Aquatic Sciences Journal, 12(3), 361-367. https://doi.org/10.29103/aa.v12i3.24729
Mahdani, M., Rizal, S., & Munandar, A. (2020). Analisis kualitas air pada kawasan budidaya keramba jaring apung di pesisir Aceh Utara. Jurnal Ilmu Lingkungan, 18(2), 310-318. https://doi.org/10.14710/jil.18.2.310-318
Nurbaiti, N., Mahdani, M., & Realita, R. (2021). Identifikasi bakteri patogen pada ikan kerapu macan (Epinephelus fuscoguttatus) di balai budidaya laut. Jurnal Akuakultur Aceh, 5(1), 23-31. https://doi.org/10.29103/jaa.v5i1.4115
Pertiwi, D. A., Sabdono, A., & Radjasa, O. K. (2023). Asosiasi bakteri patogen pada organ insang ikan kerapu di perairan tercemar. Jurnal Kelautan Tropis, 26(1), 77-85. https://doi.org/10.14710/jkt.v26i1.16241
Putra, A. N., Mustahal, M., & Syamsunarno, M. B. (2023). Respons stres fisiologis dan imunitas ikan laut akibat perubahan suhu lingkungan yang ekstrem. Jurnal Akuakultur Indonesia, 22(2), 145-154. https://doi.org/10.19028/jai.22.2.145-154
Rochmady, R., Susiana, S., & Afandy, A. (2022). Gejala klinis dan histopatologi ikan kerapu yang terserang penyakit bakterial di keramba jaring apung. Jurnal Riset Akuakultur, 17(2), 115-124. https://doi.org/10.15578/jra.17.2.2022.115-124
Sarjito, S., Desrina, D., & Haditomo, A. H. C. (2020). Agen penyebab vibriosis pada ikan kerapu lumpur (Epinephelus coioides) yang dibudidayakan di KJA. Jurnal Kelautan Tropis, 23(3), 345-352. https://doi.org/10.14710/jkt.v23i3.8610
Sugiyono. (2020). Metode penelitian kuantitatif, kualitatif, dan R&D. Alfabeta.
Syam, A. R., Haryanti, H., & Kurniastuti, E. (2024). Kerusakan histologis organ ginjal ikan kerapu lumpur akibat infeksi bakteri sistemik. Jurnal Riset Akuakultur, 19(1), 33-41. https://doi.org/10.15578/jra.19.1.2024.33-41
Tarigan, R., Salasia, S. I. O., & Tatang, M. (2023). Penerapan sistem API 20E dan API 20NE dalam identifikasi bakteri Gram negatif pada ikan air laut. Jurnal Veteriner Indonesia, 11(2), 89-98. https://doi.org/10.21157/j.vet.ind.v11i2.29153
Widanarni, W., Sukenda, S., & Wahjuningrum, D. (2022). Standardisasi metode identifikasi biokimia cepat menggunakan API Kit untuk bakteri patogen akuakultur. Jurnal Akuakultur Komersial, 4(2), 101-109. https://doi.org/10.29244/jak.4.2.101-109
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Jurnal Sains Pertanian
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian
