Molecular analysis of the 16S rRNA Gene for the identification of Shigella sp. in freshwater snail (Faunus ater)

Authors

  • Masda Admi
  • Veronika Puja Koswari
  • Winaruddin Winaruddin
  • Wahyu Eka Sari
  • Teuku Zahrial Helmi
  • Azhari Azhari
  • Faisal Jamin

DOI:

https://doi.org/10.51179/jipsbp.v7i1.3328

Keywords:

Shigella sp., 16S rRNA gene, Faunus ater, molecular

Abstract

Freshwater snails (Faunus ater) live in shallow, muddy, and sandy waters and obtain their food through a filter-feeding mechanism. These conditions make freshwater snails potentially exposed to various contaminants within their bodies, such as heavy metals and bacteria. This study aims to identify the presence of Shigella sp. in Faunus ater using 16S rRNA gene analysis. The research method was conducted through observational and laboratory exploration using a modified Carter method and 16S rRNA gene analysis. Phenotypic observations showed bacterial growth in Brain Heart Infusion Broth (BHIB) media, producing clear, round-shaped colonies on Salmonella Shigella Agar (SSA) media, and were classified as Gram-negative, short rod-shaped bacteria. The 16S rRNA gene analysis results showed that the isolate had a 98.35% similarity to Shigella flexneri strain FDAARGOS_692. It can be concluded that the molecular analysis of the 16S rRNA gene of the bacterial isolate from the freshwater snail indicates that the bacteria belong to the genus Shigella and are similar to Shigella flexneri strain FDAARGOS_692.

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Author Biographies

  • Masda Admi

    Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Veronika Puja Koswari

    Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Winaruddin Winaruddin

    Laboratorium Parasitologi, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Wahyu Eka Sari

    Laboratorium Kesmavet, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Teuku Zahrial Helmi

    Laboratorium Biokimia, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Azhari Azhari

    Laboratorium Kesmavet, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

  • Faisal Jamin

    Laboratorium Biokimia, Fakultas Kedokteran Hewan, Universitas Syiah Kuala. Kopelma Darussalam, Kota Banda Aceh, Aceh, Indonesia

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Published

2025-05-30

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Vol. 7 No. 1: May 2025

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How to Cite

Molecular analysis of the 16S rRNA Gene for the identification of Shigella sp. in freshwater snail (Faunus ater). (2025). Arwana: Jurnal Ilmiah Program Studi Perairan, 7(1), 90-97. https://doi.org/10.51179/jipsbp.v7i1.3328